Part:BBa_K801080:Design
Prepro-Thaumatin, yeast codon optimized
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Synthesized by IDT. The part is codon optimized for yeast. 3 forbidden restriction sites have been removed.
Please note, that the pre- and pro-part are split off, thus there is no possibility to add streptags or form other recombinant proteins.
Based on: http://www.ncbi.nlm.nih.gov/nuccore/121945717
Keywords:
preprothaumatin, yeast, sweetner
Abbreviations:
Design Notes
Related BioBrick: -
Cloning details:
- Designed in RFC10
Quality control measures:
- Part was totally sequenced
Backbone:
- Backbone name: pSB1C3
- Resistance: Cp
- Copynumber: high
Protein coding:
- Protein: Preprothaumatin [Nucleotide 1 to 708]
- The protein encoded is posttranslationally modified: Aa 1-22 and 229-236 are split off
Enzymatic activity: -
Cytotoxicity:
This Biobrick leads to the production of thaumatin. We investigated the toxicity of thaumatin and incubated yeast cells (strain INVSc) with 45 mM of thaumatin. We found, that thaumatin does not show any negative effect on the growth rate of yeast cells.
Safety notes:
Intellectual property: Information on patent situation: Preprothaumatin derived from Thaumatococcus daniellii
Corresponding part author/authors:
Source
Source:
- Synthesized by idt
Organism:
- Genesequence derived from Thaumatococcus daniellii
- Codonoptimized for S. cerevisiae
- Designed for the following Chassis: S. cerevisiae
- Functionality in other chassis: Should work in other chassis as well (although optimized for S. cerevisiae)
References
Literature references:
- [http://www.ncbi.nlm.nih.gov/pubmed/6327079 Pubmed: Edens L, Bom I, Ledeboer AM, Maat J, Toonen MY, Visser C, Verrips CT., 1984: Synthesis and processing of the plant protein thaumatin in yeast]
- [http://www.ncbi.nlm.nih.gov/pubmed/19489607 Pubmed: Ide N, Sato E, Ohta K, Masuda T, Kitabatake N., 2009: Interactions of the sweet-tasting proteins thaumatin and lysozyme with the human sweet-taste receptor]
- [http://www.ncbi.nlm.nih.gov/pubmed/17897626 Pubmed: Ide N, Masuda T, Kitabatake N., 2007: Effects of pre- and pro-sequence of thaumatin on the secretion by Pichia pastoris]
Database references:
- [http://www.ncbi.nlm.nih.gov/nuccore/121945717 GenBank AB265690.1: Thaumatococcus daniellii mRNA for preprothaumatin I]
- [http://http://www.ebi.ac.uk/interpro/IEntry?ac=IPR017949&query=thaumatin Interpro: Thaumatin, conserved site (IPR017949)]
- [http://www.uniprot.org/uniprot/A1IIJ1 Uniprot: Preprothaumatin I]